光致发光和温度对超弱发光的测定有很大影响。超弱发光通常包括光致发光和自发发光，但光致发光比自发发光的成分强得多[5]，因此必须消除光致发光的影响。消除光致发光有多种发光有多种方法，通常是测量暗避光处理，测量时避光。暗避光时间的长短，不同试材有所不同，萌发马铃薯种子需要5小时[25]。3T3细胞[5]和小麦幼苗、淡水蚤[2]需要1-2小时，大鼠血液[5]、CHO细胞[45]、萌发绿豆种子[5，44]都很短，仅需几分钟。温度对超弱发光也有影响，发光强度随着温度的升降而增强的减弱[24]、，将样品放入比它低几度的样品室中，5分钟后，与温度不平衡相关联的可衰减发光就只剩下了近1/4[44]。还有人认为，为了降低光电倍增管的本底，提高信噪比，需将光电倍增管冷却到300C或更低。由于超弱发光太弱，有必要增强发光以利于测定。增强超弱发光的方法很多，如引入活化剂、H2O2，以及通过电流等，现在主要是向样品中加入新配制的冷光剂[31-33，37-39，45，63]。辐照处理时可同样加入辐射增敏剂，对活细胞CHO和V79的研究表明辐射增敏剂Misonidazole能增强超弱发光强度[63]。
综上所述，经过二十余年的努力，我国在生物超弱发光，尤其是农作物的抗逆研究中，已取得了可喜成绩和进展，这为我国生物超弱发光的进一步深入研究打下了基础，对于后继者也是一个很大的策动力，但应该看到的我国超弱发光的研究领域尚存在一些问题，值得大家关注。主要集中在应用研究上，我们认为今后应强化这方面的工作，机理的研究能推动应用研究，并为应用研究提供坚实的理论基础。在应用研究方面，尤其是作物抗性研究方面有必要拓宽范围和增加深度。在抗逆研究中，目前已有的结果大都仅仅反映了超弱发光与作物抗逆性之间的定性关系，没有量化。笔者以为，每种作物都应测量尽可能多的不同抗逆性的品种，然后在超弱发光指标与抗逆性之间建立定量关系，即数学模型。有了这样的模型，对于一个抗逆性未知的样品，只要测出它的超弱发光指标，即可得出其抗逆性大小，也只有这样，超弱发光在抗逆研究中才能真正发挥作用。此外，还应将超弱发光机理与作物抗逆性机理联系起来研究，而不是仅着眼于两者的表面联系，这样才能从更深的层次探索两者的本质联系，使超弱发光及其应用研究向前跨一大步。
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