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mRNA差异显示方法研究进展 2

来源:医学杂志 2007-01-17 23:30:53 

不断完善,同时也有更新的技术问世,如DNA microarrays[24],把大量寡核苷酸片段排列在相似于一种大规模集成电路式的生物芯片上,一次可完成数量相当可观的反应,提供的信息是目前所有基因表达研究技术不可比拟的。但是,该方法在反应信号检测与数据处理等方面需要昂贵设备,比较起来,DD方法还是有其优越性,如果实验设计合理,技术应用得当,相信对分子生物学研究有帮助。

  作者单位:马庆军 100083北京医科大学第三医院骨科

  党耕町 100083 北京医科大学第三医院骨科

  宋国立 美国加州大学圣地亚哥分校骨科与生物工程系

  参考文献

  [1] Maniatis t,Goodbourn S,Fischer JA.Regulation of inducible and tissue-specific gene expression.Science,1987,236:1237.

  [2] Liang P,Pardee AB.Differential display of eukarytic messenger RNA by means of the polymerase chain reaction.Science,1992,257:967-971.

  [3] Debouck C.Differential display or differential dismay?Current Opinion biotechnology,1995,6:597-599.

  [4] Lee SW,Tomasetto C,Sager R.Positive selection of candidate tumor-suppressor genes by subtractive hybridization.Proc Natl Acad Sci uSA,1991,88:2825-2829.

  [5] Liang P,Lidia A,Pardee AB.Distribution and cloning of eukaryotic mRNA by means of differential display: refinements and optimization.Nucl Acid res,1993,21:3269-3275.

  [6] Liang P,David B,Lidia A,et al.Analysis of altered gene expression by differential display. Methods Enzymol,1995,254:304-321.

  [7] Miele G,MacRea L,McBride D,et al.Elimination of false positives generated through PCR Re-amplification of differential display cDNA.Biol techniques,1998,25:138-144.

  [8] Musholt TJ,Goodfellow PJ,Scheuman GF, et al.Differential display in primary and metastatic medullary thyroid carcinoma.J Surg Res,1997,69:94-100.

  [9] Zuo LI,Core KO,Josef EF,et al.mRNA differential display of colonic mucosa cells in ulcerative colitis.J Surg Res,1997,69:119-127.

  [10] Livesey FJ,O'Brien JA,Li B,et al.A schwann cell mitogen accompanying regeneration of motor neurons.Nature,1997,390:614-618.

  [11] Ryoo HM,Andre J,Stein JL,et al.Detection of a proliferation specific gene during development of the osteoblast phenotype by mRNA differential display. J Cell Biochem,1997,64:106.

  [12] Boden SD,Titus L,Hair G,et al.Lumbar spine fusion by local gene therapy with a cDNA encoding a novel osteoinductive protein(LMP-1).Spine,1998,23:2486.

  [13] Russell M,Utans U,Wallance AF,et al.Identification and upregulation of galactose/N-acetyl-galactosamine macrophage lectin in rat cardiac allografts with arteriosclorosis.J Clin Invest,1994,94:722-730.

  [14] Nishio Y,Aiello LP,King GL.Glucose induced genes in bovine aortic smoth muscle cells indentified by mRNA differential display.FASEB j,1994,8:103-106.

  [15] Zimmermann JW,Schultz R.Analysis of gene expression in the preimplantation mouse embryo:use of mRNA differential display.Proc Natl Acid Sci uSA,1994,91:5456-5460.

  [16] Li Z,Steven EZ,Robert EC,et al.Differential display of gene in normal and hypoplastic fetal murine lungs.J Surg Res,1998,75:66-73.

  [17] Johnson BJ,Estrada I,Shen Z,et al.Differential gene expression in response to adjunctive RhIL-2 immunotherapy in multidrug-resistant tuberculosis patients.Infect Immun,1998,66:2426-2433.

  [18] Kantorow M.Differential display detects altered gene expression between cataractous and normal human lensens.Invest Ophthalmol Vis sci,1998,39:2344-2354.

  [19] Benito EP,Prins T,Vankan JAL.Application of DDRT-PCR to the analysis of gene expression in a plant-fungus interaction.Plant Mol Biol,1996,32:947-957.

   [20] Mou L,Miller H,Li J.Improvements to the differential display method for gene analysis.Biochem Biophy Res Comm,1994,199:564-569.

   [21] Zhao S,Ooi SL,Pardee AB.New primer strategy improve precision of differential display.Biol Techniques,1995,18:842-850.

   [22] Jurecic R,Nguyen T,Belmont JW.Differential mRNA display using anchored oligo-dT and long sequence-specific primers as arbitrary primers.Trends genet,1996,12:502-504.

  [23] Stone B,Wharton W.Targeted RNA fingerprinting:the cloning of differentially-expressed cDNA fragments enriched for members of the zinc finger gene family.Nucleic Acids Res,1994,22:2612-2618.

  [24] Antoine de S,Ulrich C,Janet W,et al.Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays.Nature biotechnology,1998,16:45-48.

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