疟原虫转染技术最重要的应用就是基因敲除，但由于上述缺点迫切需要建立可诱导性转基因系统。目前认为四环素抑制子（tetracycline repressor,TetR）系统是最符合逻辑和最有前途的诱导性系统，该系统已在寄生原虫锥虫[35]和阿米巴[36]成功建立。其原理是调节基因表达的TetR与四环素抗性操纵子的调控序列结合，使RNA聚合酶不能与启动子结合，抑制下游结构基因的表达，当不同浓度的诱导物（四环素或其类似物）存在时，可不同程度地与TetR结合，使结合有四环素的TetR不能与调控序列结合，从而不同程度的开启结构基因的表达。
此外，疟原虫基因转染的效率有待于进一步提高。
作者单位：第四军医大学病原生物学教研室 西安 710032
参考文献
[1]Gardner MJ, Tettelin h, Carucci DJ, et al. Chromosome 2 sequence of the human malaria parasite plasmodium falciparum. Science, 1998,282(5391):1126～1132.
[2]Holt DC, Bourke PF, mayo M, et al.A high resolution map of chromosome 9 of Plasmodium falciparum. mol Biochem Parasitol, 1998,97(1-2):229～233.
[3]Bowman S, Lawson D, basham D, et al. The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. Nature, 1999,400(6744):532～538.
[4]Craig AG,Waters AP,Ridley rG.Malaria genome project task force: a post-genomic agenda for functional analysis. Parasitology Today,1999,15(6):211～214.
[5]Sinden RE.Malaria transfection: a new tool to study molecular function. Parasitology Today,1998,14(3):88～90.
[6]Hinnen A,Hicks JB, fink GR. Transformation of yeast. Proc Natl Acad Sci USA,1978,75(4):1929～1933.
[7]Goonewardence R,Daily J, Kaslow D,et al.Transfection of the malaria parasite and expression of firefly luciferase.Proc Natl Acad sci USA,1993,90(11): 5234～5236.
[8]Wu Y,Sifri CD,Lei hH,et al.Transfection of plasmodium falciparum within human red blood cells. proc Natl Acad Sci USA, 1995,92(4):973～977.
[9]Waters AP, Thomas AW, van Dijk MR,et al. Transfection of malaria parasites. Methods,1997,13(2):134～147.
[10]Wu Y, Kirkman LA, Wellems TE. transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine. Proc Natl Acad sci U S A, 1996,93(3):1130～1134.
[11]van Dijk MR,Waters aP,Janse CJ.Stable transfection of malaria parasite blood stages.Science,1995,268(5215):1358～
1362.
[12]Crabb BS, Cowman AF Characterization of promoters and stable transfection by homologous and nonhomologous recombination in Plasmodium falciparum. Proc Natl Acad Sci U S A,1996,93(14): 7289～7294.
[13]van der Wel AM, Tomas AM, Kocken CH, et al.Transfection of the primate malaria parasite Plasmodium knowlesi using entirely heterologous constructs.J exp Med,1997,185(8):1499～1503.
[14]Crabb BS, Triglia T, waterkeyn JG, et al. Stable transgene expression in plasmodium falciparum. Mol biochem Parasitol,1997,90(1):131～144.
[15]Sultan AA, Thathy V, nussenzweig V, et al.Green fluorescent protein as a marker in Plasmodium berghei transformation. Infect Immun,1999,67(5):2602～2606.
[16]de Koning-Ward TF, Thomas AW, Waters AP, et al.Stable expression of green fluorescent protein in blood and mosquito stages of Plasmodium berghei.Mol biochem Parasitol,1998, 97(1-2):247～252.
[17]VanWye JD, Haldar k.Expression of green fluorescent protein in Plasmodium falciparum. Mol Biochem parasitol, 1997,87(2):225～229.
[18]Chalfie M,Tu Y, Euskirchen G,et al.Green fluorescent protein as a maker for gene expression. Science,1994,263:802～805.
[19]何琪杨，张鸿卿，薛绍白.活细胞的分子探针---绿色荧光蛋白.国外医学分子生物学分册,1997,19(6):279～283.
[20]Tomas AM,van der Wel AM,Thomas AW,et al.Transfection system for animal models of malaria. Parasitology Today, 1998,14(6):245～249.
[21]Mamoun CB, Gluzman IY, Goyard S, et al.A set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparum. proc Natl Acad Sci U S A,1999,96(15): 8716～8720.
[22]Fidock DA,Wellems TE.Transfromation with human dihydrofolate reductase renders malaria parasites insnsitive to WR99210 but does not affect the intrinsic activity of proguanil. Proc Natl Acad Sci U S A,1997,94(20):10931～10936.
[23]VanWye JD,Haldar K.Expression of green fluorescent protein in plasmodium falciparum.Mol Biochem Parasitol,1997, 87(2):225～229.
[24]Waters AP,Wirth DF,Wellems TE ,et al. Transfection of malaria parasites. parasitology Today, 1996,12(4):129～132.
[25]Smith DF. Trypanosomatid transfection: stable introduction of DNA into protozoa. Parasitology Today,1990, 6(8):245～247
[26]Mamoun CB,Truong R,Gluzman I,et al.Transfer of genes into plasmodium falciparum by polyamidoamine dendrimers. Mol Biochem Parasitol,1999,103(1):117～121.
[27]Dechering KJ, Kaan AM, M
